RNA Isolation - Hard clam gill, hemos

RNA was isolated from hard clam gill (0.08g; from 20090109) and from hard clam hemos (from 20090114). One “unknown” sample (from 20090114) was also processed. Pellets were resuspended in 50uL of 0.1%DEPC-H2O. Hemo samples were pooled. Samples were spec’d.

Results: 260/280 looks OK for hemos. The gill sample has excellent 260/280 and pretty good 260/230. Samples will be reprecipitated over the weekend @ -20C according to Ambion PolyA Purist protocol in preparation for mRNA isolation.