PCR - Two new Dungan isolates

Repeat of yesterday’s PCR, but with AmpliTaq, less gDNA and 50uL rxn volume. PCR set up is here.

Gel loaded and run by Steven. Not entirely sure of the loading order. However, it doesn’t really matter…

Results: Still absolutely nothing.

UPDATE: Noticed on 20090713 that the reactions didn’t have dNTPs. Probably explains why it didn’t work!