qPCR - Abalone cDNA (07:12 set from 3/3/2009 by Lisa) and DNased RNA (from 20090623)

Now that we have a solid positive control, I’ll use the H.crach_h-1fg_intron primers to check the existing cDNA and DNased RNA. qPCR plate layout/set up is here. Anneal temp 50C.

Results: Looks like all the cDNA and DNased RNA are negative ! Finally! Will make cDNA from the DNased RNA.