Adapter Ligation - Rick’s trout fragmented control/poly I:C samples for SOLiD WTK

See the Next Gen Seq Library Database for more info. Processed the 4 samples (one set Ribominus only, one set Ribominus + PolyA enriched) according to the Agilent WTK. Briefly:

  • Speedvac’d samples to dryness

  • Resuspended RNA in 3uL H2O

  • Adapter rxn. Used all 3uL of RNA (used only 1uL of RBC Ribo only sample due to high concentration)

  • Ligation rxn

Incubated 16C for 16hrs.