qPCR - Tim’s adults gigas challenge re-DNased RNA (from today)

Performed qPCR using q18s primers on re-DNased RNA (1:100 dilution to match final concentration of template after making cDNA). qPCR set up and plate layout are here.

gDNA dilutions were used as positive controls. gDNA = BB11 (0.49ug/uL) from 20090519. Used 5uL of 1:10, 1:100 and 1:000 dilutions.

Results: re-DNase-ing the samples seems to have worked. Positive controls are the only samples to come up. Will proceed to making cDNA.