qPCR - Test Young Lab qPCR Calibration (Repeat)

This was repeated from earlier today due to the failure of the previous run, but had to use new gDNA since I ran out of the stock I had previously used. Master mix calcs and cycling params are here.


Amplification in all wells, however well E4 appears to have had some evaporation (and the effects can clearly be seen in the amplification plot below). Still getting ~3 cycle spread across the entire plate, which is disconcerting. Oddly, this is the second day where the 1st run completely failed, but the 2nd run was successful…