qPCR - Test Young Lab qPCR Calibration

This is a repeat of the two runs from yesterday, just to see if there is a correlation between the failed plates being the first of the day or not. Master mix calcs and cycling params are here (these calcs are from yesterday, but were used again for today).


Amplification in all wells. Still seeing ~3 cycle spread across the entire plate. Will work up all three successful sets of run data.