Ran qPCR with Lexie’s cDNA samples from this experiment with the following primer sets in order to better evaluate her biological reps:
QPX_SPB_F/R (SR ID: 387, 388)
LABY_A/Y (SR ID: 116, 121)
LABY was run as a potential normalizing gene. Master mix calcs are here. Plate layout, cycling params, etc. can be found in the qPCR Report (see Results). Samples were run in duplicate and were labeled according to what was written on the tops of Lexie’s cDNA tubes.
qPCR Data File (BioRad CFX96)
qPCR Report (PDF)
LABY primers worked, but the melt curves don’t look that good. I’ll let Lexie worry about the rest of the analysis.