Library Quality Assessment - C.gigas OA larvae Illumina libraries

Ran the 400ppm library and the 1000ppm library preps on a DNA1000 Assay Chip (Agilent) on the Agilent 2100 Bioanalyzer.


Data File (XAD): 2100_expert_DNA_1000_DE72902486_2015-03-02_09-18-02.xad

Electropherogram overlay of both samples:

Red = 400ppm

Blue = 1000ppm


Measurement data and parameters are here: 20150302_Bioanalyzer_Cgigas_400_1000ppm_BS-Seq

Both libraries look good; no adaptor contamination (peak would be present at ~125bp), good library sizes.

Pooled equal quantities of each library, based off the concentration values above, to prepare the sample for sequencing.

**Component** **Volume** (μL) **Quantity** (ng)
400ppm library 10 14.7
1000ppm library 1.09 14.7
Buffer EB 7.81 N/A
1% Tween20 2.1 N/A
Total 21 N/A

The pooled libraries will be submitted tomorrow to the Genomics Core Facility at the Univ. of Oregon for high-throughput sequencing (100bp, SE) on the HiSeq2500 (Illumina). Sample order #2212.