DNase Treatment - Jake’s O.lurida Ctenidia RNA (Controls) from 20150507

Since the O.lurida RNA I isolated on 20150507 showed residual gDNA via qPCR, I treated 5μg of RNA from each sample using the Turbo DNA-free Kit (Ambion/Life Technologies), following the “rigorous” protocol.


  • 50μL reactions were carried out in 0.5mL tubes

  • added 1μL of DNase to each tube

  • incubated 30mins @ 37C

  • added additional 1μL of DNased

  • incubated 30mins @ 37C

  • added 0.2 vols (10.2μL) of DNase Inactivation Reagent

  • incubated and mixed for 2mins @ RT

  • transferred 50μL of supe to sterile 1.5mL snap cap tubes

  • spec’d on Roberts Lab NanoDrop1000

Samples were stored @ -80C in Shellfish RNA Box #5 and Box #6.

DNase reaction calcs: 20150514_Jake_Oly_control_DNase_calcs


Google Spreadsheet: 20150514_DNased_RNA_Jake_Oly_controls_ODs




Overall, samples look fine. Will check for residual gDNA via qPCR.