RNA Quantification - Ronit’s C.gigas Ploidy/Dessication RNA

Last Friday, Ronit quantified 1:10 dilutions of the RNA I isolated on 20181003 and the RNA he finished isolating on 20181011, but two of the samples (D11-C, T10-C) were still too concentrated.

I made 1:20 dilutions (1uL RNA in 19uL 0.1% DEPC-treated H2O) and quantified them using the Roberts Lab Qubit 3.0, with the RNA HS assay. Used 1uL of the diluted RNA.


Qubit data (Google Sheet):

  • [20181016qubit_Cgigas_RNA](https://docs.google.com/spreadsheets/d/19S2yFspWZLClwsmu7_3osrAOA5-2RemZ-OjKB1OZpI/edit?usp=sharing)

Everything looks good. Added final concentration values (Qubit data x 20, to account for dilution factor) to Ronit’s master sheet (Google Sheet):

Will proceed with DNasing.