DNase - Ronit’s C.gigas Ctenidia RNA

Ronit finished his RNA isolations for his ctenidia samples on 20181025 & 20181101, as well as quntification (he has no notebook entry for this, at this time). However, his Qubit data can be found in these two Google Sheets:

In preparation for reverse transcription, I DNased his samples using the Turbo DNA-free Kit (Ambion) according to the manufacturer’s standard protocol.

Used 500ng of each sample.

NOTE: Sample T06 was very dilute, so performed a 60uL reaction instead of the standard 50uL reaction.

Calculations are here (Google Sheet):

Ronit’s master sheet (Google Sheet) is here:

Will check these via qPCR to verify whether or not they have any detectable gDNA.