After confirming Ronit’s DNased RNA was free of gDNA, I quantified the DNased RNA from 20181115 using the Roberts Lab Qubit 3.0 and the Qubit hsRNA Assay.
Used 1uL of each sample.
Qubit data (Google Sheet):
Well, 14 samples were too concentrated and exceeded the assay’s range, so I created 1:10 dilutions of those samples (1ul of sample in 9uL of 0.1%DEPC-treated H2O) and remeasured; again using 1uL of sample.
Admittedly, I’m not terribly surprised that happened, since they were notably lower than Ronit’s first round of RNA isolations.
I added the data to his master sheet (Google Sheet):
I will proceed with making cDNA.