MBD Enrichment - C.virginica Sheared Mantle DNA from 20190306

Yesterday, I sheared the Lotterhos C.virginica gDNA in preparation for MBD enrichment. Today, I proceeded with MBD enrichment using the MethylMiner Kit (Invitrogen) on 12 of the 24 samples. I followed the manufacturer’s protocol for input DNA >1ug - 10ug with the following modifications:

  • 2500ng of input DNA used

  • discarded washes post-DNA-binding

  • performed high-salt elution

  • elutions were stored @ -20oC until ready for EtOH precipitation next week

The full sample list and calculations are here (Google Sheet):