DNA Isolation & Quantification - C.bairdi RNA from Samples 6212_132_9 6212_334_12 6212_485_26

Isolated DNA from three samples (see Qubit spreadsheet in “Results” below for sample IDs) using the Quick DNA/RNA Microprep Kit (ZymoResearch; PDF) according to the manufacturer’s protocol for liquids/cells in RNAlater.

These samples were from RNA isolations earlier today:

Brief rundown of method:

  • Used 35uL from each RNAlater/hemocyte slurry.

  • Mixed with equal volume of H2O (35uL).

  • Retained DNA on the Zymo-Spin IC-XM columns at 4oC for isolation after RNA isolation.

  • DNA was eluted in 15uL H2O

DNA was quantified on the Roberts Lab Qubit 3.0 using the 1x DNA High Sensitivity Assay (Invitrogen), using 1uL of each sample.


RESULTS

Qubit results (Google Sheet):

Samples were stored at -80oC in:

Rack 15, 4, 5 in C.bairdi gDNA Box #2

Sample ID [DNA] (ng/uL)
6212_485_26 28.8
6212_132_9 19.7
6212_334_12 23.6