RNA Isolation and Quantification - P.generosa Hemocytes from Shelly

Shelly asked me to isolate RNA from some P.generosa hemocytes (GitHub Issue) that she had.

RNA was isolated using the Quick DNA-RNA Microprep Plus Kit (ZymoResearch), according to the “Cells” protocol with the following notes/changes:

  • All samples were lysed with 400uL of DNA/RNA Lysis buffer

  • All samples were DNased using DNase aliquots from 20200127 (prepped by Kaitlyn Mitchell).

Samples were quantified using 1uL of sample with the Qubit RNA HS Assay (Invitrogen) on the Roberts Lab Qubit 3.0.

Two samples (022H and 044H) were too concentrated, so a 1:10 dilution was prepared and the two samples were re-quantified.

See the RESULTS section below for Qubit data and summary table of sample concentrations/yields.


RESULTS

All samples were stored in the -80oC in Shellfish RNA Box #8, slots E7 - F3.

Qubit results:

Sample Concentration(ng/uL) Elution_vol(uL) Yield(ng)
021 H 176 15 2640
022 H 382 15 5730
041 H 102 15 1530
044 H 580 15 8700
14 H 194 15 2910
15 H 124 15 1860