Sam’s Notebook

University of Washington - Fishery Sciences - Roberts Lab

Posts - Page 2 of 132

Data Received - C.gigas Diploid-Triploid pH Treatments Ctenidia WGBS from ZymoResearch

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Today we received the whole genome bisulfite sequencing (WGBS) from the [24 C.gigas diploid-triploid samples subjected to different pH that were submitted 20200824](https://robertslab.github.io/sams-notebook/2020/08/24/Sample-Submitted-C.gigas-Diploid-Triploid-pH-Treatments-Ctenidia-to-ZymoResearch-for-WGBS.html. The lengthy turnaround time was due to a bad lot of reagents, which forced them Zymo to find a different manufacturer in order to generate libraries.

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Sample Submission - M.magister MBD BSseq Libraries for MiSeq at NOAA

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Earlier today I quantified the libraries with the Qubit in preparation for sample pooling and sequencing. Before performing a full sequencing run, Mac wanted to select a subset of the libraries based on the experimental treatments to have an equal representation of samples. She also wanted to do a quick run on the MiSeq at NOAA to evaluate how well libraries map and to make sure libraries appear to be sequencing at relatively equal levels.

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