RNA Quantification - Ronit’s C.gigas DNased RNA from 20181115

RNA quantification
Qubit 3.0
Qubit hsRNA
Crassostrea gigas
pacific oyster
diploid
triploid
dessication
DNased RNA
2018
Author

Sam White

Published

November 20, 2018

After confirming Ronit’s DNased RNA was free of gDNA, I quantified the DNased RNA from 20181115 using the Roberts Lab Qubit 3.0 and the Qubit hsRNA Assay.

Used 1uL of each sample.

RESULTS

Qubit data (Google Sheet):

Well, 14 samples were too concentrated and exceeded the assay’s range, so I created 1:10 dilutions of those samples (1ul of sample in 9uL of 0.1%DEPC-treated H2O) and remeasured; again using 1uL of sample.

Admittedly, I’m not terribly surprised that happened, since they were notably lower than Ronit’s first round of RNA isolations.

I added the data to his master sheet (Google Sheet):

I will proceed with making cDNA.