We received the RNAseq data from the RNA that was sent out by Grace on 20200212.
Sequencing is 150bp PE.
Grace has a Google Sheet that describes what the samples constitute (e.g. ambient/cold/warm, infected/uninfect, day, etc.)
Genewiz report:
Project | Sample ID | Barcode Sequence | # Reads | Yield (Mbases) | Mean Quality Score | % Bases >= 30 |
---|---|---|---|---|---|---|
30-343338329 | 72 | ACTCGCTA+TCGACTAG | 27,249,335 | 8,175 | 34.16 | 85.82 |
30-343338329 | 73 | ACTCGCTA+TTCTAGCT | 25,856,008 | 7,757 | 33.87 | 84.36 |
30-343338329 | 113 | ACTCGCTA+CCTAGAGT | 31,638,462 | 9,492 | 32.38 | 77.77 |
30-343338329 | 118 | ACTCGCTA+GCGTAAGA | 29,253,455 | 8,776 | 33.50 | 82.62 |
30-343338329 | 127 | ACTCGCTA+CTATTAAG | 27,552,329 | 8,266 | 33.14 | 81.13 |
30-343338329 | 132 | ACTCGCTA+AAGGCTAT | 27,518,702 | 8,256 | 34.86 | 88.87 |
30-343338329 | 151 | ACTCGCTA+GAGCCTTA | 33,430,314 | 10,029 | 35.01 | 89.35 |
30-343338329 | 173 | ACTCGCTA+TTATGCGA | 33,262,459 | 9,979 | 34.45 | 87.06 |
30-343338329 | 178 | GGAGCTAC+TCGACTAG | 29,495,389 | 8,849 | 35.01 | 89.62 |
30-343338329 | 221 | GGAGCTAC+TTCTAGCT | 25,902,415 | 7,771 | 34.76 | 88.40 |
30-343338329 | 222 | GGAGCTAC+CCTAGAGT | 53,808,137 | 16,142 | 30.90 | 71.11 |
30-343338329 | 254 | GGAGCTAC+GCGTAAGA | 16,771,613 | 5,031 | 35.14 | 90.03 |
30-343338329 | 272 | GGAGCTAC+CTATTAAG | 27,818,893 | 8,346 | 33.30 | 81.70 |
30-343338329 | 280 | GGAGCTAC+AAGGCTAT | 61,008,799 | 18,303 | 30.85 | 70.86 |
30-343338329 | 294 | GGAGCTAC+GAGCCTTA | 28,539,233 | 8,562 | 35.12 | 90.04 |
30-343338329 | 334 | GGAGCTAC+TTATGCGA | 25,916,895 | 7,775 | 34.98 | 89.39 |
30-343338329 | 349 | GCGTAGTA+TCGACTAG | 32,868,756 | 9,861 | 33.53 | 82.69 |
30-343338329 | 359 | GCGTAGTA+TTCTAGCT | 27,274,149 | 8,182 | 34.96 | 89.20 |
30-343338329 | 425 | GCGTAGTA+CCTAGAGT | 66,224,932 | 19,867 | 29.54 | 65.13 |
30-343338329 | 427 | GCGTAGTA+GCGTAAGA | 18,918,640 | 5,676 | 33.31 | 80.87 |
30-343338329 | 445 | GCGTAGTA+CTATTAAG | 30,745,388 | 9,224 | 33.07 | 80.83 |
30-343338329 | 463 | GCGTAGTA+AAGGCTAT | 19,531,145 | 5,859 | 34.27 | 86.08 |
30-343338329 | 481 | GCGTAGTA+GAGCCTTA | 50,592,084 | 15,178 | 31.92 | 75.59 |
30-343338329 | 485 | GCGTAGTA+TTATGCGA | 26,010,208 | 7,803 | 34.63 | 87.48 |
Confirmed that SFTP transfer from Genewiz to owl/nightingales/C_bairdi/
was successful:
RESULTS
Output folder:
Will update the nightingales Google Sheet with the appropriate info shortly.