qPCR - C.gigas polyIC

2023
Miscellaneous
Author

Sam White

Published

July 26, 2023

Using cDNA from 20230721, performed qPCR on the following primer sets selected by Steven (GitHub Issue), along with Cg_Actin as a potential normalizing gene:

SRID Primer
1170/1 Cg_Actin
1830/1 Cg_DICER
1172/3 Cg_GAPDH
1832/3 Cg_IRF2
1834/5 Cg_SACSIN
1828/9 Cg_VIPERIN
1826/7 Cg_cGAS
1383/4 Cg_citrate_synthase

qPCR calcs:

Reactions were run on white, low-profile, 96-well qPCR plates (USA Scientific). A total of two plates were run. One was run on a CFC Connect (BioRad) and the other on a CFX96 (BioRad). See the qPCR Report in the RESULTS section below for cycling params, plate layouts, etc.

All samples were run in singular. Normally duplicates/triplicates are run. However, due to time and sample constraints, samples were just run in singular. No template controls were run in duplicate.

RESULTS

Brief note(s) on results:

  • Samples T2 and T15 seem to come up earlier than all other samples in almost all of the primer sets.

qPCR Results file (CSV)

qPCR Data file (QPCRD - CFX Maestro required)

qPCR Report (PDF)

PLOTS

SRID Primer Amplification Plot Melt Curve
1170/1 Cg_Actin Cg_Actin amplification plot Cg_Actin melt plot
1830/1 Cg_DICER Cg_DICER amplification plot Cg_DICER melt plot
1172/3 Cg_GAPDH Cg_GAPDH amplification plot Cg_GAPDH melt plot
1832/3 Cg_IRF2 Cg_IRF2 amplification plot Cg_IRF2 melt plot
1834/5 Cg_SACSIN Cg_SACSIN amplification plot Cg_SACSIN melt plot
1828/9 Cg_VIPERIN Cg_VIPERIN amplification plot Cg_VIPERIN melt plot
1826/7 Cg_cGAS Cg_cGAS amplification plot Cg_cGAS melt plot
1383/4 Cg_citrate_synthase Cg_citrate_synthase amplification plot Cg_citrate_synthase melt plot