Steven asked me to generate cDNA (GitHub Issue) from Crassostrea gigas (Pacific oyster) RNA, as part of Matt George’s polyIC project. I had previously quantified RNA from five diploid and five triploid samples (Notebook), but the qPCR results (Notebook) didn’t reveal much. Plus, the diploids and triploids weren’t the same treatment types (i.e. the diploids were poly:IC-injected, whereas the triploids were controls - so they weren’t really comparable). So, Steven asked that I find comparable set of samples so that we’d have either diploid controls or triploids which were poly:IC-injected. Looking in the freezer box for samples (Google sheet), I found four diploid MgCl2 control RNA samples. Before starting reverse transcription, I felt it would be best to quantify the RNA using the Qubit 3.0 using the Broad Range Assay. Steven and I discussed which samples to use earlier this week.
Used 1uL of each sample.
See Results section below for sample/tube labels.
RESULTS
- 20230816-qubit-cgig-polyIC-RNA (Google Sheet)
All samples had ample amount of RNA. Will proceed with reverse transcription.