Reverse Transcription - C.gigas Lifestage Carryover Seed and Spat

INTRO

After isolating RNA earlier today (Notebook) for the project-gigas-carryover/lifestage_carryover (GitHub repo), I proceeded with reverse transcription for those samples with sufficient yields of RNA.

MATERIALS & METHODS

Reverse transcription was run using 100ng of each sample, using M-MLV reverse transcriptase (Promega) and oligo dT primers (Promega), according to the manufacturer’s protocol (PDF). Oligo dT primers and M-MLV reverse transcriptase amounts were adjusted to maintain the proper ratio, relative to the amount of input RNA.

Calculations:

• 20240319-cgig-carryover-spat-seed-cDNA-calcs (Google Sheet)

RESULTS

cDNA was stored in the -20^oC freezer in FTR 213.

Box layout:

• Lifestage Carryover C.gigas cDNA Box 1

Box with blue label: “Lifestage Carryover, C.gigas cDNA Box #1”