RNA Isolation - V. tubiashii from challenge (see 20081216)

2008
Miscellaneous
MICROBExpress Kit
NanoDrop1000
RNA isolation
RNA quantification
TriReagent
Vibrio tubiashii
Author

Sam White

Published

December 22, 2008

Added 10mL TriReagent to 2 x 50mL pellets (5.63 x 10^11 total bacteria; see calcs on 20081219 from the control samples collected on 20081219. Added 10mL TriReagent to 1 x 50mL pellet (1.835 x 10^12 total bacteria; see calcs on 20081219 from the V.tubi + oyster samples collected on 20081219. Scaled rest of RNA protocol to match. Resuspended pellets in 100uL 0.1%DEPC-H2O. Samples were NanoDropped.

external image 20081222%20RNA%20SJW.PNG

The V. tubi + gigas sample was eventually diluted to contain 400uL (see final reading for that sample above).

RNA samples were precipitated O/N @ -20C according to Ambion protocol.