Ran PCR with GoTaq on the new Dungan isolate gDNA from yesterday. PCR set up is here. 53C annealing temp.
Lane 1 - Hyperladder
Lane 2 - 17t
Lane 4 - H5
Lane 6 - 12t
Lane 7 - Hyperladder
Lane 8 - 1.5t
Lane 10 - 1.2t
Lane 12 - 11t
lane 13 - Hyperladder
Lane 14 - 13t
Lane 16 - 19t
Lane 18 - H2O
Lane 19 - Hyperladder
Lane 20 - H2O
Results: All the bands present are a bit larger than 400bp. However, the bottom band in the H5 sample is larger than any band present in any other samples. Additionally, the largest band in the H5 sample is between 800-1000bp. Bands were cut out from H5 (two bands: Top, Bottom), 1.5t, 1.2t, 13t. These will be purified and sequenced.
It’s also interesting to note that the bands present in this gel are found in the same samples as the last time this analysis was done. See Kevin’s Notebook, 20090212.