Repeat of PCR from 20090403, but using AmpliTaq and 50C annealing temp. PCR set up is here.
Lane 1 - Hyperladder
Lane 2 - 19t
Lane 3 - 100bp ladder
Lane 4 - 13t
Lane 6 - 17t
Lane 8 - 1.5t
Lane 9 - Hyperladder
Lane 10 - 1.2t
Lane 12 - 11t
Lane 13 - 100bp ladder
Lane 14 - H5
Lane 16 - 12t
Lane 17 - 100bp Ladder
Lane 18 - H2O
Lane 19 - H2O
Lane 20 - Hyperladder
Results: Nothing amplified! Possibly due to age of polymerase (?); over a year old. Will wait to repeat for new primers to arrive (EukA/B).