Did PCR with new Euk primers designed by Steven. Should be one step higher taxonomically. PCR set up is here. Aneal temp 50C.
Lane 1 - Hyperladder
Lane 2 - 19t
Lane 4 - 13t
Lane 6 - 17t
Lane 7 - 100bp ladder
Lane 8 - 1.5t
Lane 10 - 1.2t
Lane 11 - Hyperladder
Lane 12 - 11t
Lane 14 - H5
Lane 15 - 100bp ladder
Lane 16 - 12t
Lane 18 - H2O
Lane 19 - H2O
Laen 20 - Hyperladder
Results: The new EukA/B primers worked wonderfully. The brightest band in each lane was excised and purified using Millipore DA spin columns. These will be stored and sequenced at a later date.