Steven had me re-PCR the old Dungan isolates with the new EukA/B primers. Anneal temp 50C. [PCR set up here (bottom half of sheet)(./20090410-01.jpg) .
NOTE: Sample #30 was not in the rack of tubes that Steven gave to me.
Results:
Steven had me re-PCR the old Dungan isolates with the new EukA/B primers. Anneal temp 50C. [PCR set up here (bottom half of sheet)(./20090410-01.jpg) .
NOTE: Sample #30 was not in the rack of tubes that Steven gave to me.
Results: