Steven had me re-PCR the old Dungan isolates with the new EukA/B primers. Anneal temp 50C. [PCR set up here (bottom half of sheet)(https://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20090410-01.jpg) .
NOTE: Sample #30 was not in the rack of tubes that Steven gave to me.
Results: