Used 3 sets of reverse primers:
Bay_Actin_Rv0
Bay_Actin_Rv2
Sea_Actin_Rv2
Primers were slected based on information from Steven’s notebook (#8, 12/30/2007-1/3/2008). Anneal temp 53C.
PCR set up here . Plate layout here .
Samples were run out by Steven the following day.
Gel 1 of 3
Gel 2 of 3
Gel 3 of 3
Results: