Prepared cDNA using an equal amount of RNA from all samples (442.6ng)(https://spreadsheets.google.com/ccc?key=0AmS_90rPaQMzcHdyU1d0MDVMLWphMFdTOHUwVHFqWnc&hl=en). This amount was based on using the maximum allowable volume of RNA for the RT rxn AND the sample with the lowest [RNA] (08:3-20; 24.59ng/uL). cDNA was prepared according to the Promega MMLV RT recommendations. Here is the work up for the cDNA rxns. cDNA was stored @ -20C.
