DNase Treatment (Rigorous!) - Mac’s gigas RNA/Re-DNased RNA from 20090507 & 20090508, respectively

2009
PROPS
Author

Sam White

Published

May 12, 2009

Followed the rigorous protocol for Ambion’s Turbo DNA-free protocol for the following RNAs:

BB#11-20

DH#11-20

Used 10ug of RNA (200ng/uL) in 50uL as directed. Here are the calcs for FF and DH.

Followed standard protocol on DNased samples from 20090508:

BB#1-10

DH#1-10

The standard protocol should be fine for these samples, since the procedure worked on Friday for some of them.