RNA Isolation - Martha’s Vineyard (MV) hemocytes

Samples were pelleted at 100g, 4C for 10mins. The supe was mostly removed, leaving ~50uL of supe above the pellets. RNA was isolated according to the TriReagent protocol from the following samples:

3226:

• A7

• A10

• A15

• A17

• A20

• A22

• A26

• 3326:

• B1

• B8

• B10

• B13

• B14

• B22

• B26

• B29

RNAs were resuspended in 10uL of 0.1% DEPC-H2O. They will be DNase treated.