Set up qPCR with Cv_18s_F/R primers on the following samples that had previously come up negative in both reps using the diluted (1:20) cDNA that Mac had made specifically for the 18s runs:
3326A13
2100B07
3219A06
2100B15
3326A11
2100B12
2100A03
Plate layout/PCR set up is here. This is a second rep of these samples.
Results: Waters are clean. However, the following samples still are negative for an 18s amplicon: