PCR of MIE-14v just to make sure that we can’t get a product from this sample, despite NanoDrop readings suggesting that there’s no DNA. Used both LABY and Euk primer sets. PCR set up is here. Anneal temp 50C.
Lane 1 - 100bp Ladder
Lane 2 - Euk
Lane 3 - Euk H2O
Lane 4 - Euk H2O
Lane 5 - Euk H2O
Lane 6 - LABY
Lane 7 - LABY H2O
Lane 8 - LABY H2O
Lane 9 - LABY H2O
Lane 10 - 100bp Ladder
Results: Nothing, as expected. Need to devise a new method of isolating gDNA from these “problem” isolates.