Performed qPCR using q18s primers on DNased RNA (1:100 dilution to match final concentration of template after making cDNA). qPCR set up and plate layout are here.
gDNA dilutions were used as positive controls. gDNA = BB11 (0.49ug/uL) from 20090519. Used 5uL of 1:10, 1:100 and 1:000 dilutions.
Results: All samples, including positive controls, came up negative! Realized that I accidentally used the EF1 primers which will NOT amplify gDNA. And, to top it off, this was BEFORE going to seminar (i.e. before having any beers).