This is an exact repeat of the PCR from yesterday, due to inconsistencies between repeated PCRs from yesterday and earlier today. Here’s yesterday’s workup
Results:
Gel Loading (from left to right):
Opsin Primers (lanes 2-10), Rhodopsin Primers (same loading order, lanes 12-20)
1 - 100bp ladder
2 - retina
3 - fin
4 - 4th arm
5 - dorsal mantle center
6 - dorsal mantle side
7 - ventral mantle center
8 - ventral mantle side
9 - H2O
10 - H2O
Opsin Primers
Single bands in retina, fin and ventral mantle center samples. Negative controls are clean. Bands will be excised and stored @-20C in Sam’s “Purified Inserts” box for eventual sequencing. Tubes are labelled with a “2” to differentiate them from the gel run earlier today.
Rhodopsin Primers
Single bands in retina and fin. Double bands in ventral mantle center sample. Second band is very faint is ~700bp. Negative controls are clean. Bands, including the faint 700bp VMC, will be excised and stored @-20C in Sam’s “Purified Inserts” box for eventual sequencing. Tubes are labelled with a “2” to differentiate them from the gel run earlier today.