Duplicates of earlier qPCRs.
Primers: Cg_HIF1 and IL17 Iso D.
The previous version of IL17 primers used (IL17 internal) were NOT the ones used for the paper. The IL17 Iso D are the correct primers and are the same ones that Tim previously used on the juvenille gill samples.
qPCR set up and plate layout can be found here.
Results:
Duplicates of earlier qPCRs.
Primers: EF1 and IL17 Iso D.
The previous version of IL17 primers used (IL17 internal) were NOT the ones used for the paper. The IL17 Iso D are the correct primers and are the same ones that Tim previously used on the juvenille gill samples. qPCR set up and plate layout can be found here.
Results: