ePCR SOLiD Libraries - Abalone CC, CE pools and yellow perch CT SOLiD libraries (from 20100408)

2010
Miscellaneous
Author

Sam White

Published

April 12, 2010

Emulsion PCR (ePCR) was performed for the above three mentioned SOLiD libraries using 1.5pM (180 pg/uL) of cDNA, according to the ABI “full scale” ePCR protocol. ePCRs were stored @ 4C until ready for the emulsion breaking step.

Amounts of cDNA used to make dilutions (in 1x Low TE Buffer) of 180pg/uL:

CC (13.8ng/uL): 1.3uL in 100uL

CE (23.01ng/uL): 1.56uL in 200uL

CT (63.8ng/uL): 1.41 in 500uL