Restriction Digest - Oly Oyster gDNA-01 for RAD Sequencing (from 20141029)

2014
Olympia oyster reciprocal transplant
Author

Sam White

Published

October 31, 2014

Samples required two days of drying for all samples to fully dry down.

Reconstituted all samples in 20uL of PCR H2O.

Performed restriction digests:

Incubated at 37C for 90mins and then inactivated enzyme @ 80C for 20mins. Incubation was done in thermal cycler using heated lid. Plate was stored @ -20C.