RAD Sequencing - Oly Oyster gDNA-01 RAD Library (from 20141110)

2014
Olympia oyster reciprocal transplant
Samples Submitted
library prep
olympia oyster
Ostrea lurida
RAD-seq
Author

Sam White

Published

November 17, 2014

Prepared 10nM of the library in total volume of 20uL of Buffer EB (Qiagen) + 0.1% Tween-20, according the University of Oregon’s Genomic’s Core.

Based on a calculator provided by their site, 10nM was equivalent to 2.93ng/uL. Diluted 3.13uL of the library (18.7ng/uL) in 16.87uL of Buffer EB + Tween 20.

Shipped FedEx Standard Overnight on wet ice for 100bp single end Illumina sequencing.