Bisulfite NGS Library Prep - LSU C.virginica Oil Spill MBD Bisulfite DNA and Emma’s C.gigas Larvae OA Bisulfite DNA (continued from yesterday)

Continued library prep from yesterday. Set up Library Amplification according to the protocol. The samples received the following Barcode Indices:

• HB2 - 1 (ATCACG)

• HB5 - 2 (CGATGT)

• HB16 - 3 (TTAGGC)

• HB30 - 4 (TGACCA)

• NB3 - 5 (ACAGTG)

• NB6 - 6 (GCCAAT)

• NB11 - 7 (CAGATC)

• NB21 - 12 (CTTGTA)

• 1A1 - 2 (CGATGT)

• 1A2 - 1 (ATCACG)

• 6A1 - 4 (TGACCA)

• 6A2 - 5 (ACAGTG)

• 103B1 - 6 (GCCAAT)

• 103B2 - 7 (CAGATC)

• 105A4 - 12 (CTTGTA)

• 105A5 - 11 (GGCTAC)

Due to differences in input DNA quantities, samples were run with different numbers of thermal cycles.

13 thermal cycles were run for the following samples:

• 1A1

• 105A4

• 105A5

22 thermal cycles were run for the following samples:

• HB2

• HB5

• HB16

• HB30

• NB3

• NB6

• NB11

• NB21

• 1A2

• 6A1

• 6A2

• 103B1

• 103B2

Samples were quantified with 1uL of each sample using the Quant-iT dsDNA BR Kit (Invitrogen). Used 5uL of each standard and standards were run in duplicate.

Results: