The two pooled bisulfite-treated DNA samples (400ppm and 1000ppm) from 20150115 were used to prepare bisulfite Illumina libraries with EpiNext Post-Bisulfite DNA Library Preparation Kit (Illumina) (Epigentek) (PDF). Changes to the manufacturer’s protocol:
Samples were transferred to 1.5mL snap cap tubes for all magnetic bead steps in order to fit in our tube magnets.
Stopped after End Repair step (prior to magnetic bead clean up). Samples stored @ -20C