Isolated RNA from [Jake’s Ostrea lurida ctenidia samples that had been subjected to mechanical stress (from 20150422)(https://heareresearch.blogspot.com/2015/04/4-22-2015-heatmechanical-shock.html).
Despite the indication in this notebook, the samples had not been previously homogenized in RNAzol RT. I thawed the samples, homogenized them and followed the RNAzol RT protocol for total RNA isolation. Here’s the list of samples:
42215 NM1 1
42215 NM1 2
42215 NM1 3
42215 NM1 4
42215 NM1 5
42215 NM1 6
42215 NM1 7
42215 NM1 8
RNA was resuspended in 50μL of 0.1%DEPC-H2O and stored @ -80C in the original box they came from.