RNA Quantification - O.lurida 1hr post-mechanical heat stress DNased RNA

2015
Olympia oyster reciprocal transplant
ctenidia
DNased RNA
gill
mechanical stress
NanoDrop1000
olympia oyster
Ostrea lurida
RNA quantification
Author

Sam White

Published

August 6, 2015

DNased RNA from 20150727 was quantified using the Roberts Lab NanoDrop1000.

Results:

The 260/280 ratios don’t look great, but that is most likely due to the DNase treatment. The DNase that’s added to each sample isn’t actually removed, so that additional protein will skew the 260/280 ratios. Will proceed with qPCR to check for any residual gDNA in these samples.

(http://eagle.fish.washington.edu/Arabidopsis/20150806_Jake_oly_mech_stress_DNasedRNA_ODs.JPG)

(http://eagle.fish.washington.edu/Arabidopsis/20150806_Jake_oly_mech_stress_DNasedRNA_plots_01.JPG)

(http://eagle.fish.washington.edu/Arabidopsis/20150806_Jake_oly_mech_stress_DNasedRNA_plots_02.JPG)