Continued the extractions that Steven began yesterday (dead link - onsnetwork.org/halfshell/2015/09/15/upon-improving-extractions/) and this morning (dead link - onsnetwork.org/halfshell/2015/09/16/running-the-numbers/) using the E.Z.N.A. Mollusc DNA Kit (Omega Bio-Tek) after the RNase treatment @ 70C.
Samples were cooled to RT (~10mins)
Added 300μL of 100% EtOH to each (equivalent to the volume of aqueous phase Steven recovered from each sample)
Followed manufacturer’s protocol
Eluted all samples with 50μL of Elution Buffer
Spec’d on Roberts Lab NanoDrop1000
Results:
(http://eagle.fish.washington.edu/Arabidopsis/20150916_gDNA_Oly_RAD_ODs_01.JPG)
(http://eagle.fish.washington.edu/Arabidopsis/20150916_gDNA_Oly_RAD_ODs_02.JPG)
(http://eagle.fish.washington.edu/Arabidopsis/20150916_gDNA_Oly_RAD_plots_01.JPG)
(http://eagle.fish.washington.edu/Arabidopsis/20150916_gDNA_Oly_RAD_plots_02.JPG)
DNA looks absolutely pristine and has amazing yields.
Will check gDNA integrity via agarose gel tomorrow.
Data has been entered into the master spreadsheet for this project: Ostrea lurida Project Master Tissue Sample List