Agarose Gel - Geoduck & Olympia oyster gDNA Integrity Check

2015
Geoduck Genome Sequencing
Olympia Oyster Genome Sequencing
gDNA
gel
geoduck
O'geneRuler DNA Ladder Mix
olympia oyster
Ostrea lurida
Panopea generosa
Author

Sam White

Published

September 17, 2015

Ran a 0.8% agarose, 1x modified TAE gel (w/EtBr) with geoduck and Olympia oyster gDNA that was precipitated earlier today. Used 5μL of each sample (~500ng).

Results:

[caption id=“” align=“alignleft” width=“255”](http://eagle.fish.washington.edu/Arabidopsis/20150917_gel_gDNA_geoduck_oly.jpg) Geoduck gDNA on left. Oly gDNA on right.[/caption]

(https://raw.githubusercontent.com/sr320/LabDocs/master/protocols/Commercial_Protocols/ThermoFisher_OgeneRuler_DNA_Ladder_Mix_F100439.jpg)

Overall, the DNA still looks very good. Slight smearing (indicating slight degradation), but the high molecular weight band is very prominent. Will fill out the necessary BGI forms and ship samples out on Monday.