Pooled the gDNA samples from earlier today & from 20151002.
The pooled volume = 260μL
Quantified on the Roberts Lab NanoDrop1000.
Results:
(http://eagle.fish.washington.edu/Arabidopsis/20151005_gDNA_geoduck_pool_ODs.JPG)
(http://eagle.fish.washington.edu/Arabidopsis/20151005_gDNA_geoduck_pool_plots.JPG)
Besides the weird peak at 240nm, everything looks great - perfect 260/280 & 260/230 ratios.
Yield = 75.92μg
This gDNA has been RNase treated, so the concentration should be semi-accurate. Regardless, even if there’s a discrepancy of 50%, this should provide enough additional DNA for BGI to work with.
Will run on gel to evaluate integrity and then send to BGI.