DNA Sonication - Oly gDNA for MBD

2015
MBD Enrichment for Sequencing at ZymoResearch
bioanalyzer
Bioruptor 300
electropherogram
hc1_2B
hc1_4B
hc2_15B
hc2_17
hc3_1
hc3_10
hc3_11
hc3_5
hc3_7
hc3_9
Hood Canal
olympia oyster
Ostrea lurida
Oyster Bay
sonication
ss2_14B
ss2_18B
ss2_9B
ss3_14B
ss3_15B
ss3_16B
ss3_20
ss3_3B
ss3_4B
ss5_18
Author

Sam White

Published

November 19, 2015

In preparation for MBD enrichment, fragmented Olympia oyster gDNA with a target size of ~350bp.

Genomic DNA samples were isolated and provided to us by Katherine Silliman at UIC. Selected samples will compare Hood Canal (HC) and Oyster Bay (SS, South Sound) populations.

Used the Seeb Lab’s Bioruptor 300 (Diagenode) sonicator.

(http://eagle.fish.washington.edu/Arabidopsis/20151119_bioruptor_settings.JPG)

After sonication, samples were run on a the Seeb Lab’s 2100 Bioanalyzer (Agilent) on DNA 12000 chips.

Results:

HOOD CANAL SAMPLES

(http://eagle.fish.washington.edu/Arabidopsis/Bioanalyzer%20Data/20151119_bioanalyzer_oly_hood_canal_all_electropherograms.jpg)

OYSTER BAY SAMPLES

(http://eagle.fish.washington.edu/Arabidopsis/Bioanalyzer%20Data/20151119_bioanalyzer_oly_oyster_bay_all_electropherograms.jpg)

More detailed analysis (including average fragment size for each samples) will be coming soon…