We opted to go with ZymoResearch for this project because they could do the bisulfite treatment and finish the sequencing by the end of January.
Submitted the following 18 Ostrea lurida MBD-enriched gDNA samples to ZymoResearch for bisulfite treatment and subsequent Illumina sequencing (50bp, single read):
| hc1_2B |
| hc1_4B |
| hc2_15B |
| hc2_17 |
| hc3_1 |
| hc3_10 |
| hc3_11 |
| hc3_5 |
| hc3_7 |
| hc3_9 |
| ss2_14B |
| ss2_18B |
| ss2_9B |
| ss3_14B |
| ss3_15B |
| ss3_16B |
| ss3_20 |
| ss3_3B |
| ss3_4B |
| ss5_18 |
The samples will be bisulfite treated, Illumina libraries constructed, multiplexed, and this multiplexed library will be sequenced three times.