Assembly Comparison - Oly Assemblies Using Quast

2017
Olympia Oyster Genome Sequencing
Author

Sam White

Published

November 14, 2017

I ran Quast to compare all of our current Olympia oyster genome assemblies.

See Jupyter Notebook in Results section for Quast execution.

Results:

Output folder: https://owl.fish.washington.edu/Athaliana/quast_results/results_2017_11_14_12_30_25/

Heatmapped table of results: https://owl.fish.washington.edu/Athaliana/quast_results/results_2017_11_14_12_30_25/report.html

Very enlightening!

BEST OF:

Largest Contig: redundans_sjw_02 (322,397bp) Total Length: soap_bgi_01 & pbjelly_sjw_01 (697,528,655bp) Total Length (>=50,000bp): redundans_sjw_03 (17,006,058bp) N50: redundans_sjw_03 (17,679bp)

Interesting tidbit: The pbjelly_sjw_01 assembly is EXACTLY the same as the soap_bgi_01. Looking at the output messages from that PB Jelly assembly, one can see why. The messages indicate that no gaps were filled on the BGI scaffold reference! That means the PB Jelly output is just the BGI scaffold reference assembly!

Jupyter Notebook (GitHub): 20171114_swoose_oly_assembly_comparisons_quast.ipynb