RNA Quantification - Ronit’s C.gigas DNased RNA from 20181115

2018
Miscellaneous
Author

Sam White

Published

November 20, 2018

After confirming Ronit’s DNased RNA was free of gDNA, I quantified the DNased RNA from 20181115 using the Roberts Lab Qubit 3.0 and the Qubit hsRNA Assay.

Used 1uL of each sample.

RESULTS

Qubit data (Google Sheet):

Well, 14 samples were too concentrated and exceeded the assay’s range, so I created 1:10 dilutions of those samples (1ul of sample in 9uL of 0.1%DEPC-treated H2O) and remeasured; again using 1uL of sample.

Admittedly, I’m not terribly surprised that happened, since they were notably lower than Ronit’s first round of RNA isolations.

I added the data to his master sheet (Google Sheet):

I will proceed with making cDNA.